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Investigating the effect of oil spills
on the environment and public health.
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Louis J. Guillette

Medical University of South Carolina

Department of Obstetrics and Gynecology

171 Ashley Avenue
Charleston, SC  29425  

Project List:

Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure

Year 3-5 Investigator Grants (RFP-II)Role: Co-Principal Investigator, Outreach Team, Outreach Point of Contact

Publications & Presentations:

Journal Articles - 4

2016

Bowers, R. R., Temkin, A. M., Guillette, L. J., Baatz, J. E., & Spyropoulos, D. D. (2016). The commonly used nonionic surfactant Span 80 has RXRα transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers. General and Comparative Endocrinology, 238, 61-68.
Temkin, A. M., Bowers, R. R., Magaletta, M. E., Holshouser, S., Maggi, A., Ciana, P., Guillette, L. J., Bowden, J. A., Kucklick, J. R., Baatz, J. E., & Spyropoulos, D. D. (2016). Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPAR <b>γ</b> Activity <i>in Vitro</i> and <i>in Vivo</i> : Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen. Environmental Health Perspectives, 124(1), 112-119.

2015

Kohno, S., Bernhard, M. C., Katsu, Y., Zhu, J., Bryan, T. A., Doheny, B. M., Iguchi, T., & Guillette, L. J. (2015). Estrogen Receptor 1 (ESR1; ERα), not ESR2 (ERβ), Modulates Estrogen-Induced Sex Reversal in the American Alligator, a Species With Temperature-Dependent Sex Determination. Endocrinology, 156(5), 1887-1899.

2014

Kohno, S., Parrott, B. B., Yatsu, R., Miyagawa, S., Moore, B. C., Iguchi, T., & Guillette Jr., L. (2014). Gonadal Differentiation in Reptiles Exhibiting Environmental Sex Determination. Sexual Development, 8(5), 208-226.

Conference Presentations - 48

2016

Brumbaugh, A. W., C.; Guillette, L. S., & D.D.; Kohno, S. (2016). Obesogenic activity of oil dispersant corexit 9500 in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Cantu, T. B., & J.A.; Guillette, L. J. (2016). Inflammation in sentinel species and the curious case of pansteatitis at Loskop Dam, South Africa. In 25 Years of Endocrine Disruption Research: Past Lessons and Future Directions
Cantu, T. B., J.A.; Lowers, R. G., & L.J (2016). Assessing inflammation and oxidative stress in wildlife species affected by pansteatitis in South Africa. In Gordon Research Conference, Environmental Endocrine Disruptors
Cantu, T. B., J.A.; Lowers, R. G., & L.J (2016). Examination of predictive parameters for pansteatitis in Nile crocodiles and prey species along the Olifants River, South Africa: The use of targeted lipidomics for wildlife health assessments. In 24th International meeting of the Crocodile Speacialist Group
Cantu, T. B., J.A.; Lowers, R. G., & L.J (2016). Investigation of eicosanoids and fatty acids in pansteatitis-affected Mozambique tilapia at Loskop Dam, South Africa. In American Society for Mass Spectrometry, 2016
Cantu, T. B., J.A.; Temkin, A. S., & D.D.; Guillette, L. J. (2016). Eicosanomics: Novel Approaches to Investigate the Effect of Oil/Dispersant Exposure on Eicosanoid Biosynthesis in Sentinel Species. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Cantu, T. B., J.A.; Temkin, A. S., & D.D.; Guillette, L. J. (2016). Life Lessons From Wildlife And Lou: The Tale Of Pansteatitis And Use Of Novel Eicosanomic Techniques To Assess Inflammation In Sentinel Species. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Kohno, S. N., R.B.; Nishizawa, H. G., L.J.; Kitai, T. S., & T (2016). Transgenerational alteration of estrus cycle proportion in F1 female mice obtained from F0 males neonatally exposed to 17?-hydroxyprogesterone caproate. In Annual Meeting of Society for Maternal Fetal Medicine
McNabb, N. M., S.; Iguchi, T. S., D.D.; Guillette, L. K., & S (2016). Investigating estrogenicity of oil dispersant COREXIT® EC9500A in the American alligator and diamondback terrapin. In Annual Meeting of Society for Maternal Fetal Medicine
McNabb, N. S., D.D.; Guillette, L. K., & S (2016). Investigating estrogenicity and developmental effects of the dispersant COREXIT® EC9500A in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
McNabb, N. S., D.D.; Guillette, L. K., & S (2016). Investigating Estrogenicity Of COREXIT In The American Alligator: Learning In The Field And Lab From Lou Guillette. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
McNabb, N. W., C.E.; Spyropoulos, D. G., & L.J.; Kohno, S. (2016). Investigating effects of COREXIT and CWAF on sex determination in the American alligator. In Palmetto Alligator Research and Management Symposium.
Spyropoulos, D. T., A.M.; Cantu, T. M., N.A.; Williams, C. B., R.R.; Bowden, J. K., J.R.; Kohno, S. B., & J.E.; Guillette, L. (2016). From Oiled Stem Cells to Pregnant Women: Methodically Navigating Between Complex Environments and Human Health. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Temkin, A. B., R.R.; Guillette, L. B., J.A.; Kucklick, J. B., & J.E.; Spyropoulos, D. (2016). In vivo Exposure and Stem Cell Transcriptomics To Determine If Dioctyl Sodium Sulfosuccinate (DOSS) Is A Bona Fide Obesogen. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Temkin, A. B., R.R.; Magaletta, M. H., S.; Maggi, A. C., P.; Guillette, L. B., J.A.; Kucklick, J. B., & J.E.; Spyropoulos, D. (2016). DOSS in Corexit and in Laxatives: Finding a Poster Child for Encouraging Movement in Dispersant and Obesity Sciences. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Temkin, A. B., R.R.; Sojka, C. A., M.; Guillette, L. B., J.A.; Kucklick, J. B., & J.E.; Spyropoulos, D. (2016). Rapid and sensitive assay screening reveals multiple potential hormone disrupting activities in MC252 crude oil and different dispersants. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Williams, C. G., L.J.; Spyropoulos, D. K., & S (2016). Estrogenic effects of CWAF exposure on sex determination in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016
Williams, C. G., L.J.; Spyropoulos, D. K., & S (2016). Effects of exogenous estradiol on gonadal sex determination in the American alligator during in vitro culture of the isolated gonad-adrenal-mesonephric complex. In Annual Meeting of the Society for Integrative and Comparative Biology
Williams, C. S., D.D.; Guillette, L. K., & S (2016). Lessons in research and beyond from Lou Guillette - assessing the impacts of CWAF on sex determination in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016

2015

Bernhard, M. S., D.D.; Guillette, L. K., & S (2015). Can tributyltin be used as a positive control for obesogenic exposure in the American alligator (Alligator mississippiensis)?. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015
Finnell, B. B., & J.; Guillette, L. (2015). A Snapshot of Perfluoroalkyl Acids in Southeastern Populations of American Alligator. In 2015 Medical University of South Carolina, HML Poster Session of the Summer Undergraduate Research Program: Endocrine Disrupting Compounds, the Environment and Human Health.
Finnell, B. B., & J.; Guillette, L. (2015). A Snapshot of Perfluoroalkyl Acids in Southeastern Populations of American Alligator. In 2015 Medical University of South Carolina Summer Undergraduate Research Program Oral Presentations
Guillette, L. (2015). Environmental factors influence the epigenetics of the developing vertebrate gonad. In European Community EpiConception Program -Invited Plenary Speaker
Kitai, T. N., H.; Iguchi, T. G., L.J.; Kohno, S. S., & T (2015). Reproductive study of f1 mice obtained from F0 mice neonatally exposed to 17?-hydroxyprogesterone caproate. In 63rd NIBB Conference: Environment to Bioresponse
Kohno, S. M., N.A.; Williams, C. S., D.D.; Guillette, L. P., & B.B (2015). Long-term influences of environmental factors on sex determination and differentiation in the American alligator. In 63rd NIBB Conference: Environment to Bioresponse
McNabb, N. S., D.D.; Guillette, L. K., & S (2015). Effects Of COREXIT 9500 As A Potential Endocrine Disruptor On Sex Determination Of The American Alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015
McNabb, N. G., L.J.; Spyropoulos, D. K., & S (2015). Investigating estrogenicity and developmental effects of the dispersant COREXIT in the American alligator and diamondback terrapin. In 19th Annual College of Charleston Graduate Program in Marine Biology Student Research Colloquium
McNabb, N. M., S.; Iguchi, T. S., D.D.; Guillette, L. K., & S (2015). Investigating estrogenicity and developmental effects of the dispersant COREXIT in the American alligator and diamondback terrapin. In 63rd NIBB Conference: Environment to Bioresponse
Perez-Viscalles, J. C., T.M; Bowden, J. G., & L.J (2015). Eicosanoid activity in the Chorioallantoic membrane of the developing chicken. In College of Charleston NSF REU MIMES undergraduate research symposium.
Temkin, A. B., R.; Baatz, J. G., & L.; Spyropoulos, D. (2015). Evaluating the endocrine disrupting potential of crude oil and dispersant via transactivation assay screens. In SETAC North America 36th Annual Meeting
Temkin, A. B., R.; Baatz, J. G., & L.; Spyropoulos, D. (2015). Identification and characterization of putative obesogens in crude oil and dispersants used in the deepwater horizon oil spill. In SETAC North America 36th Annual Meeting
Temkin, A. B., R.; Baatz, J. G., L.J.; Kohno, S. S., & D.D (2015). Keynote Speaker: Empowering Awareness of Environmental Impacts on Women's Health - From Wildlife and Stem Cells to the Clinic and Personal Choices. In Carolina Women’s Health Research Forum
Temkin, A. B., R.R.; Guillette, L. S., & D.D (2015). RFP-II: Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015
Temkin, A. B., R.R.; Magaletta, M. H., S.; Maggi, A. C., P.; Guillette, L. B., J.A.; Kucklick, J. B., & J.E.; Spyropoulos, D. (2015). Predicting Long Term Impacts of Oil/dispersant Exposures on Human Health and Higher Trophic Organisms: Obesogenicity. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015
Williams, C. G., L.J.; Spyropoulos, D. K., & S (2015). Estrogenic Effects Of CWAF Exposure In Vitro On Sex Determination Of The American Alligator. In 19th Annual College of Charleston Graduate Program in Marine Biology Student Research Colloquium

2014

Barrett, N. T., A.; Ellisor, D. K., S.; Guillette, L., & ; Spyropoulos, D. (2014). Histological Analysis of Adipose Tissue in the Tail of American Aliigators Exposed to Tributyltin in ovo. In MUSC Marine Biomedicine & Environmental Sciences Program, Summer Undergraduate Research Program Student Colloquium
Barrett, N. T., A.; Kohno, S. G., L.J, ;. S., & D.D (2014). Histological Analysis of Adipose Tissue in the Tail of American Aliigators Exposed to Tributyltin in ovo. In Summer Undergraduate Research Program, Final Presentation Day
Bernhard, M. K., S.; Guillette, L., & ; Spyropoulos, D. (2014). Effects Of Ppar?/Rxr? Signaling Via In Ovo Tributyltin Exposure On Development Of The American Alligator, Alligator Mississippiensis. In College of Charleston Marine Biology Graduate Program Student Research Colloquium 2014
Guillette, L. (2014). The Fragile Fetus: Sentinel Species and Environmental Health. In Annual Undergraduate Research symposium, USC Upstate - Keynote Speaker
Guillette, L. (2014). Embryonic Origins of Adult Disease: Environmental Contaminants and the Need for 'Green'er Chemistry. In Annual International ‘High Polymer Research Group’ Meeting - Keynote Speaker
Guillette, L. (2014). Environmental contaminants and wildlife sentinel species: gene expression and epigenetic regulation of gonadal development. In Society of Environmental Toxicology and Chemistry; SETAC Asia/Pacific and Australasia Converence 2014
Guillette, L. P., B.; Bowden, J. K., & S (2014). Endocrine Disrupting Contaminants and the Developing Reproductive System. In Society for the Study of Reproduction 2014 ; Fertility: A Global Challenge
Kohno, S. S., T.; Miyagawa, S. N., R.; Iguchi, T. G., & L.J (2014). Neonatal exposure to a synthetic progestin, 17?-hydroxyprogesterone caproate suppressed estrogenic responsiveness in transcriptome of female reproductive tract in adult mice. In Gordon Research Conferences, Mammalian Reproduction
McCoy, J. P., B.; Guillette, L., & ; Spyropoulos, D. (2014). Do warmer eggs yield hyper-masculinized alligator hatchlings?. In MUSC Marine Biomedicine & Environmental Sciences Program, Annual Student Research Open House
McNabb, N. K., & S.; Guillette, L. (2014). Effects of COREXIT 9500 As A Potential Endocrine Disruptor On Sex Determination Of The American Alligator. In College of Charleston Marine Biology Graduate Program Student Research Colloquium 2015
Patton, M. T., A.; Ellisor, D. K., S.; Guillette, L., & ; Spyropoulos, D. (2014). Using Molecular Markers to Rapidly Characterize Adipogenic Stem Cell Differentiation. In 2014 Annual Meeting of SICB

2013

Ellisor, D. C., J.A, C., T.M.; Kohno, S. B., J.E.; Guillette, L. S., & D.D (2013). Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2013
Ellisor, D. C., J.A, C., T.M.; Kohno, S. B., J.E.; Guillette, L. S., & D.D (2013). Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2013